Volume 8 Issue 6 | June 2014 issue
Table of Contents
8(6) 2014
8(6) 2014
Southern Cross Publishing Group©2014
AUSTRALIA
AUSTRALIA
Australian Journal of Crop Science | June 2014
Volume 8 Number 6 2014
Identification of Cercospora leaf spot resistance among fenugreek accessions and characterization of the pathogen
Rajib Prasad, Surya Acharya*, Scott Erickson, James Thomas
Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada
Summary
To identify resistant germplasms and to morphologically characterize the disease pathogen on fenugreek plants, 53 accessions adapted to western Canada were screened by artificially inoculating plants with 1Χ104 spores ml-1 conidial suspensions of C. traversiana in growth cabinets. Out of these, nine accessions were characterized as resistant (R) or moderately resistant (MR) and the rest were susceptible. A more detailed study with 20 selected (9 resistant and 11 other) accessions using the artificial inoculation method revealed that the accessions were significantly (p = 0.01) different in their reaction to the disease.
Pages 822-830 | Full Text PDF
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Assessment of popping ability of new tropical popcorn hybrids
Phumelele Jele, John Derera*, Muthulisi Siwela
School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa
KwaZulu-Natal Department of Agriculture and Environmental Affairs, South Africa
Summary
The objectives of the study were to determine variability among hybrids for popping quality traits, gene action involved in conditioning of quality traits in popcorn hybrids and relationships among quality traits in popcorn. Random crosses were generated among inbred lines resulting in 119 experimental F1 hybrids with adequate seed. A commercial hybrid P618 grown under the same conditions was used as a standard check. Significant variability was observed among hybrids for popping quality traits (p=0.05). The study identified 15 hybrids with superior popping quality. The hybrid 11POPH13 gave the highest flake volume (1 288 cm3) and the highest popping fold (25.75).
Pages 831-839 | Full Text PDF
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The effects of different concentrations of NAA on oil palm (Elaeis guineensis) embryoid cultures and phytosterols production
Zati Hanani Termizi*, Norrizah Jaafar Sidik, Tarmizi Ahmad Hashim, Norizan Ahmat
Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Malaysia
Tissue Culture Department, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi Bandar Baru Bangi, 43000 Kajang, Malaysia
Summary
This study aimed to investigate the effect of NAA concentrations on further improvement of oil palm (Elaeis guineensis) embryoid cultures of three selected Malaysian Palm Oil Board (MPOB) clones (PL 213, PL 209 and PL 220) and phytosterols production. The results indicated that PL 213 gave the highest mean of fresh weight (55.3±3.4 g) and number of shoots (123±4) in MS medium without NAA (control) followed by clones PL 209 and PL 220. There is a significant difference of clone and NAA concentration on the fresh weight of embryoid cultures but there is no significant effect of NAA concentration on the number of shoots.
Pages 840-847 | Full Text PDF
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Histological changes on regeneration in vitro culture of Date palm (Phoenix dactylifera) leaf explants
Ola H. Abd El Bar*and Maiada M. El Dawayati
Agriculture Botany Department, Faculty of Agriculture, Ain Shams University, 68 Hadayek Shoubra 11241, Cairo, Egypt
The Central Laboratory of Date Palm Researches and Development, Agriculture Research Center, Cairo, Egypt
Summary
A tissue culture experiment was conducted to investigate the effect of some growth regulators and two different ages of in vitro leaves of date palm c.v. sakoty on regeneration process. Leaves were cultured on Murashige and Skoogs medium (MS) supplemented with each or combined with the following growth regulators 4 mg l-1 2,4-D, 40 mg l-1 NAA, 2 mg l-1BA, (2 mg1 -1BA+ 40 mg l-1 NAA), (2mgl-1 BA+ 4 mg l-1 2,4-D) and (2 mg l-1BA+40 mg l-1 NAA+4 mg l-1 2,4-D). The results showed that in vitro young leaf explants treated with 2 mg1 -1 BA was more efficient in inducing direct somatic embryos and subsequent plantlets growth in a short time duration ,i.e.8-10 months.
Pages 848-855 | Full Text PDF
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Diagnosing nitrogen and sulfur status in marandu palisadegrass via non-destructive and destructive sampling methods
Karina Batista and Francisco Antonio Monteiro*
Instituto de Zootecnia, Secretaria de Agricultura e Abastecimento, Nova Odessa, SP, Brazil
Soil Science Department, Escola Superior de Agricultura Luiz de Queiroz, University of Sγo Paulo, Piracicaba, SP, Brazil
Summary
The objective of this study was to diagnose nitrogen and sulfur status in Brachiaria brizantha cv. Marandu via destructive and non-destructive sampling methods by growing this forage grass with different combinations of nitrogen and sulfur in a Quartzipsamment soil with low organic matter content. The results showed that diagnostic leaves of nitrogen-deficient marandu palisade grass plants had SPAD values of 16 to 20 and nitrogen concentrations of 11 to 14 g kg-1. Nitrogen-sufficient grasses had a SPAD value of =40-45 and nitrogen concentrations of >29.2 g kg-1. The sulfur status of grasses depended on their supply of nitrogen. Diagnostic leaves of plants treated with nitrogen but not sulfur had SPAD values of 17-28, sulfur concentrations of 0.6-1.2 g kg-1, sulfur-sulfate concentrations of 56-84 mg kg-1, and N:S ratios of up to 80:1.
Pages 856-865 | Full Text PDF
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Epistasis effects and inheritance of harvest index, drought and heat-resistance related traits in groundnut (Arachis hypogaea L.)
Chuni Lal*, Krishna Hariprasanna, Bharat Chikani, Harsukh Gor
Directorate of Groundnut Research, P. B. 5, Junagadh 362001, Gujarat, India
Directorate of Sorghum Research, Rajendranagar, Hyderabad 500030, India
Summary
Genetic control of harvest index (HI), and traits related to drought and heat resistance was studied following a triple test cross mating design. The inheritance pattern revealed importance of epistasis in the manifestation of all the traits. Partitioning of total epistasis to its component parts detected additive Χ additive (i) type epistasis for HI, and additive Χ dominance (j) + dominance Χ dominance (l) type for ?13C, however, for SCMR and RCI both i and j+l types of epistasis were important with the preponderance of the former. In two lines (JL 24 and PBS 12160), epistatic deviations were observed for SLA also when these were detected with the t-test.
Pages 866-872 | Full Text PDF | Supplementary data
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Bioassimilation behaviour of tomato seedling cultivars under different sources of artificial light
Eva Marνa Almansa, Antonio Espνn, Rosa Marνa Chica, Marνa Teresa Lao*
Agronomy Department of Higher Engineering School, University of Almeria, Ctra. Sacramento s/n, La Caρada de San Urbano, 04120, Almerνa, Spain
Higher Technical School of Civil Engineering,University of Granada, Campus Universitario de Fuentenueva, Edificio Politιcnico C/ Severo Ochoa s/n, 18071, Granada, Spain
Engineering Department of Higher Engineering School, University of Almerνa,Ctra. Sacramento s/n, La Caρada de San Urbano, 04120, Almerνa, Spain
Summary
The behaviour of fifteen tomato cultivars under different lights was carried out and quality of seedlings (biomass, plant structure, water status, carbohydrates starvation), was studied. The lamps used were compact fluorescent (184 W·m-2), high efficiency fluorescent (140 W·m-2) and tubular dons bulb fluorescent (108 W·m-2). Cultivars did not show the same behaviour under light treatments. The maximum response in biomass production of cultivars is obtained under high efficiency fluorescent treatment (16.56 W·m-2 PAR). For all cultivars, the relationship between total fresh weight and total dry weight presented the lowest value under high efficiency fluorescent treatment (low ratio, grate hardening). Starch quantity was higher than sugars for all treatments. Ikram cultivar has the greatest capacity to synthesize carbohydrates in all treatments.
Pages 873-880 | Full Text PDF
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Evaluation of some important woody plant species against wood destroying activity of honey fungus
Rahele Soltantoyeh*, Alireza Dalili, Ali Borhani
Damghan Islamic Azad University, Damghan, Iran
Agricultural and Natural Resources Research Center of Mazandaran, P.O. Box 48175-556, Sari, Iran
Summary
Armillaria mellea is one of the most important pathogens of plant species in horticultural and forest regions of Iran. In order to management of Armillaria root rot disease by tolerant plant species, resistant of 11 woody plants species was investigated against Armillaria mellea. The data analysis proved that Armillaria mellea isolates A23 and A1 caused the highest and lowest level of wood destroying activity with 11.82 and 7.11% wood weight loss, respectively. The tested plant indicated significant difference against the fungus. Citrus aurantium L., Ficus carica L., and Olea europaea L. with 19.00, 16.25 and 15.85% wood weight loss showed susceptible reaction to A. mellea. However, Zelkova carpinifolia Dippel with 1.68% wood weight loss were introduced as most tolerant species to A. mellea which followed by Prunus divaricate Ledeb. and Diospyrus lotus L., with 5.6 and 7.46% of wood weight loss, respectively.
Pages 881-886 | Full Text PDF
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Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.)
Sandra Mansur Scagliusi
EMBRAPA - Brazilian Agricultural Research Corporation, National Wheat Research Center, Passo Fundo - RS, 99001-970, Brazil
Summary
The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect.
Pages 887-894 | Full Text PDF
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Anatomy of vegetative organs and seed histochemistry of Physalis peruviana L.
Filipe Almendagna Rodrigues*, Joyce Dσria Rodrigues Soares, Renata Alves Lara Silva, Edwaldo dos Santos Penoni, Moacir Pasqual, Fabrνcio Josι Pereira, Evaristo Mauro de Castro
Tissue Culture Laboratory of the Department of Agriculture at Lavras Federal University, 37200-000, Lavras, Minas Gerais, Brazil
Laboratory of Plant Anatomy in the Department of Biology of Lavras Federal University, 37200-000, Lavras, Minas Gerais, Brazil
Summary
Physalis peruviana L. (Solanaceae) is a plant species whose fruits can be classified as fine fruits, which is similar to other species such as blueberry, raspberry, cherry, blackberry and pitaya. The objectives of the present study were to evaluate the type of reserve that occurs in seeds and anatomically characterize vegetative organs of Physalis peruviana L. (physalis). Histochemical tests and anatomical characterization were performed on the vegetative organs such as root, stem and leaf. Physalis seeds showed the presence of lipid reserves. The exposure of physalis to the east-west axis promoted thickening of the leaf midrib and blade and promoted the development of secondary xylem and phloem in the stem. Thus, the leaves on this axis appeared to show traits that favor greater radiation tolerance, which is a trait typical of sun leaves. The root of physalis has a uniseriate epidermis with the exodermis just below the epidermis.
Pages 895-900 | Full Text PDF
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Phenology, phyllochron, and gas exchanges in frigo and fresh strawberry (Fragaria Χ ananassa Duch.) plants of cv. Albion
Rosiani Castoldi da Costa*, Eunice Oliveira Calvete, Heloνsa Ferro Constβncio Mendonηa, Ana Paula Cecatto
University of Passo Fundo, Faculty of Agronomy and Veterinary Medicine, Campus I-Barrio San Josι - BR 285 - Km 171, CEP :99052-900, 611 Pillar Box - Passo Fundo, Rio Grande do Sul, Brazil
Summary
Aiming to identify a possible precocity in frigo and fresh strawberries plants of cv. Albion, in greenhouse, the cycle and the rate of leaf appearance were evaluated and the photosynthetic behavior of these plants was analyzed. Relating phenology evaluations and phyllochron determinations, frigo plants were more precocious than fresh plants. However, even frigo plants presenting a lower leaf emission rate, the thermal sum needed for the emission of successive leaves is lower (93.9 ΊC day leaf-1). Therefore, our study allows the producers, who want to sell strawberries in offseason, use frigo plants. However, fresh plants are recommended for those who cultivate in the traditional season.
Pages 901-908 | Full Text PDF
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Variation for infection response to Bipolaris sorokiniana and identification of trait specific sources in barley (Hordeum vulgare L.) germplasm
Tejveer Singh, Vinod K. Mishra*, Lal C. Prasad, Ramesh Chand
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi 284003, India
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Summary
The aim of present study was to evaluate un-adapted, exotic and indigenous germplasm of barley to identify useful genetic diversity for spot blotch resistance and other related traits to barley breeders. A total 124 genotypes were evaluated for spot blotch resistance and yield components, for three consecutive years at Varanasi, India. Based on Area Under Disease Progress Curve (AUDPC) score of spot blotch, three germplasm accessions (BCU422, BCU1204 and BCU5092) were found resistant to virulent isolate of spot blotch disease.
Pages 909-915 | Full Text PDF | Supplementary data
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QTL for grain yield under water stress and non-stress conditions over years in rice (Oryza sativa L.)
Sunil Kumar Verma*, Ritu Ravi Saxena, Ravi Ratna Saxena, Mary Suchita Xalxo, Satish Balkrishna Verulkar
Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur-492 012 Chhattisgarh, India
Department of Agriculture Statistics and Social Science, Indira Gandhi Krishi Vishwavidyalaya, Raipur-492 012 Chhattisgarh, India
Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur- 492 012 Chhattisgarh, India
Summary
A RIL population (F12 and F13 generation) developed from a cross between the two indica cultivars, Danteshwari (high yielding variety) and Dagad deshi (drought tolerant local land race). The population was phenotyped under combination of absolute rainfed (RF), terminal stage drought (TSD) and irrigated (I) conditions with direct seeding and transplanting during 2011 and 2012 to identify QTL for grain yield. Genotyping of all 122 RIL lines were done using 162 polymorphic SSR and HvSSR markers. The phenotypic and genotypic data was analyzed using QTL cartographer 2.5 and QTL IciMapping 3.2. The recombinant inbred lines exhibited significant interaction with conditions. In general, the lines performing better under stress were poor under non-stress condition; however, two lines (#17 and 57) performed well under all sets of conditions.
Pages 916-926 | Full Text PDF | Supplementary data
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Validation of SSR markers linked to the rust and late leaf spot diseases resistance in diverse peanut genotypes
Kanagee Navinbhai Gajjar, Gyan Prakash Mishra, Thankappan Radhakrishnan*, Sneha Meramanbhai Dodia, Arulthambi Luke Rathnakumar, Narendra Kumar, Santosh Kumar, Jentilal Ramjibhai Dobaria, Abhay Kumar
Directorate of Groundnut Research, Post Box No. 05, Junagadh- 362 001, Gujarat, India
Summary
In the present investigation, 22 SSR markers linked to rust and LLS diseases resistance were tested on 95 diverse genotypes for marker validation, of which 16 SSRs could be validated. Among rust resistant varieties and germplasm lines, nearly perfect marker validation was recorded but for 30 wild Arachis species, marker validation was very poor. Maximum numbers of rust and LLS linked-markers were found to be present on the linkage group 03 (eight SSRs) followed by linkage group 04 (three SSRs) which is an important step towards identification of more closely linked markers to the rust and LLS resistance QTLs and its cloning in future.
Pages 927-936 | Full Text PDF | Supplementary data
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Review article
Diversity assessment of sorghum germplasm and its utilization in genetic analysis of quantitative traits-A review
Tariq Shehzad* and Kazutoshi Okuno
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Japan 305-8572
Alliance for Research on North Africa (ARENA), University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Japan 305-8572
Summary
In this review, we describe the importance of genetic diversity in sorghum germplasm and its utilization in crop improvement through genetic analysis of important traits. The use of two different approaches of QTL mapping, namely family based linkage mapping and linkage disequilibrium based association mapping are discussed. This article surveys how QTL-based methods contribute to a better understanding of genetics mechanism involved in controlling phenotypic traits of interest. Some of the case studies of QTL mapping, controlling important traits in sorghum, are also reported in this review.
Pages 937-944 | Full Text PDF | Supplementary data
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Arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing fungus (PSF) on tolerance of beach plum (Prunus maritima) under salt stress
Zai Xueming*, Hao Zhenping, Zai Yu, Zhang Huanshi, Qin Pei
Horticulture Department, Jinling Institute of Technology, 130 village centers Qixia District, Nanjing, 210038, PR China
School of Pharmacy, Nanjing University of Chinese Medicine, 22 Xian-lin Avenne, Nanjing, 210046, PR China
Halophyte Research Laboratory, Nanjing University, 22 Han-kou Road, Nanjing, 210093, PR China
Summary
The synergic effect of an arbuscular mycorrhizal fungus (AMF) and a phosphate-solubilizing fungus (PSF) on the salt-tolerance of beach plum (Prunus maritima) grown in pots was explored. Pot experiments in greenhouse were carried out in one year beach plum inoculated with AMF (Funneliformis mosseae) or/and PSF (Mortierella sp. SM-1) under 1% NaCl stress. Salinity dramatically increased Na+ concentrations, but decreased K+ contents and K+/ Na+ in shoot and root significantly. And meanwhile, salinization reduced growth (root and shoot dry weight,total phosphate contents, chlorophyll contents) and biological soil quality (soil available phosphate contents and phosphatase enzyme activities).
Pages 945-950 | Full Text PDF
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Effect of Liquinox and Rosasol-N fertilizers on vegetative growth of phalaenopsis seedlings after transplantation into ex vitro conditions
I Gede Ketut Adiputra
Department of Biology, Faculty of Natural Science, University of Hindu Indonesia, Denpasar, Bali, Indonesia
Jl. Sangalangit, Tembau, Penatih, Denpasar, Bali, Indonesia
Summary
Ex vitro experiments were conducted to study the effect of NAA containing fertilizer (Liquinox) and high N containing fertilizer (Rosasol-N) on growth rate, chlorophyll content and viability of phalaenopsis seedlings previously micro propagated in vitro. This study found that during 46 days period of growth in ex vitro conditions, seedlings added Rosasol-N underwent both substantial decreases in growth rate and chlorophyll contents. In Liquinox added plants, whilst growth rate was slightly decreased, chlorophyll content was found substantially decreased.
Pages 951-956 | Full Text PDF
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Nitrogen use efficiency is associated with chlorophyll content in Brazilian spring wheat
Cristiano Lemes da Silva, Giovani Benin*, Elesandro Bornhofen, Eduardo Beche, Matheus Henrique Todeschini, Anderson Simionato Milioli
Agronomy Graduate Program, Federal University of technology - Paranα, Pato Branco - PR, CEP 85501-970, Brazil
Agronomy Course, Federal University of technology - Paranα, Pato Branco - PR, CEP 85501-970, Brazil
Summary
The objective of this study was to evaluate the general and specific combining ability (GCA and SCA) of agronomic traits associated with NUE components in a diallel cross. Six wheat parents and fifteen F2 populations were planted in the field in a randomized block design with three replications. The relative chlorophyll content was measured in flag leaves at the post-anthesis stage, while agronomic traits and NUE components were evaluated at physiological maturity. Additive and non-additive genetic effects were identified controlling NUE components.
Pages 957-964 | Full Text PDF
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Sugarcane cultivars present differential susceptibility to herbicides ametryn and trifloxysulfuron-sodium
Alexandre Ferreira da Silva, Leandro Galon, Germani Concenηo*, Ignacio Aspiazϊ, Evander Alves Ferreira, Siumar Pedro Tironi, Antonio Alberto da Silva
Brazilian Agricultural Research Corporation (EMBRAPA) Maize and Sorghum, Sinop - MT, Brazil
Federal University of the South Border (UFFS), Erechim - RS, Brazil
Brazilian Agricultural Research Corporation (EMBRAPA) Western Region Agriculture, Dourados - MS, Brazil
State University of Montes Claros (UNIMONTES), Janaϊba - MG, Brazil
PNPD-CAPES Fellowship, Jequitinhonha and Mucuri Valleys Federal University, Diamantina - MG, Brazil
Federal University of Alagoas, Maceiσ - AL, Brazil
Federal University of Viηosa, Viηosa - MG, Brazil
Summary
We aimed this study to evaluate the influence of the selective herbicides ametryn, trifloxysulfurom-sodium and its commercially available mixture on the growth of sugarcane cultivars. The results showed that trifloxysulfuron-sodium causes lower impact on all variables; therefore, being more selective to all cultivars. The label dose (1.0x), equivalent to 2,000 g ha-1 of ametryn and 22.5 g ha-1 of trifloxysulfuron-sodium is considered as a threshold between deficient weed control (in lower doses) and excessive crop toxicity (in higher doses). RB867515 and RB855113, respectively, were the most and least tolerant cultivars to both herbicides. In highly infested planting fields, farmers usually prefer to grow the most tolerant sugarcane cultivar when applying ametryn or trifloxysulfurom-sodium.
Pages 965-972 | Full Text PDF
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Proteome analysis of wheat (Triticum aestivum) for the identification of differentially expressed heat-responsive proteins
Ranjeet Ranjan Kumar*, Gyanendra Pratap Singh, Suneha Goswami, Himanshu Pathak, Raj Deo Rai
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India Pin 110012
Division of Genetics, Indian Agricultural Research Institute, New Delhi, India Pin 110012
CESCRA, Indian Agricultural Research Institute, New Delhi, India Pin 110012
Summary
We performed an extensive identification of heat-responsive proteins in control (22°C) and heat shock (HS) treated (42°C, 2 h) leaf samples of HD2985 cultivar of wheat at milky-ripe stage using 2-DE coupled with MALDITOF/MS, nLC/MS and MS2. Orbitrap was used because of its highest rate of true protein identification and speedy processing of samples. We identified 47 and 38 unique protein spots with 19 differentially expressed in control and heat shock (HS) treated samples.
Pages 973-986 | Full Text PDF | Supplementary data PDF | Supplementary data XLS
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Transferability of microsatellite loci in Coffea canephora
Ludymila Brandγo Motta, Taνs Cristina Bastos Soares*, Maria Amιlia Gava Ferrγo, Eveline Teixeira Caixeta, Ronald Martins Pereira Jϊnior, Rodrigo Monte Lorenzoni
Doutoranda do Programa de Pσs-Graduaηγo em Produηγo Vegetal, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Departamento de Farmαcia e Nutriηγo, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Embrapa Cafι/Incaper, Vitσria , ES, 29052-010, Brasil
Embrapa Cafι, BIOAGRO, BIOCAFΙ, Universidade Federal de Viηosa, Viηosa, MG, 36570-000, Brasil
Graduando em Agronomia, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Summary
We tested the heterologous amplification of 71 microsatellite markers, originally developed for Coffea arabica, in Coffea canephora. Heterologous amplification occurred with 38 of the 71 primers. Approximately 52.63% of the primers used were polymorphic; 35% of the primer sets were classified as non-informative, 50% as moderately informative and 15% as highly informative. The loci evaluated by SSRCa 088, M793, SSRCa 054, SSRCa 085, CM5, M25, CFGA54 were low informative; SSRCa 084, SSRCa 052 and SSRCa 040 were highly informative. The validated primer sets can be used in molecular studies for different purposes once they were efficient in the genotypes discrimination.
Pages 987-991 | Full Text PDF | Supplementary data
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Quality control to seeding systems and densities in peanut crop
Cristiano Zerbato*, Carlos Eduardo Angeli Furlani, Murilo Aparecido Voltarelli, Rafael Scabello Bertonha, Rouverson Pereira da Silva
Department of Rural Engineering, Universidade Estadual Paulista-UNESP/FCAV, Prof. Paulo Donato Castellane Access way, 14884-900 - Jaboticabal, SP-Brazil
Summary
The objective of this study was to use Statistical Process Control (SPC) to evaluate the quality of the operation of peanut seeding systems and densities. The parameters included the number of days to emergence, the longitudinal distribution of seedlings, the initial stand of plants and the crop yield. The seeding systems maintained a quality standard for the average number of days to seedling emergence. Compared to the longitudinal distribution of the seedlings in the control group, the mechanised seedings presented higher percentage of flawed spacing, which did not affect the crop yield.
Pages 992-998 | Full Text PDF
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Volume 8 Number 6 2014
Identification of Cercospora leaf spot resistance among fenugreek accessions and characterization of the pathogen
Rajib Prasad, Surya Acharya*, Scott Erickson, James Thomas
Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada
Summary
To identify resistant germplasms and to morphologically characterize the disease pathogen on fenugreek plants, 53 accessions adapted to western Canada were screened by artificially inoculating plants with 1Χ104 spores ml-1 conidial suspensions of C. traversiana in growth cabinets. Out of these, nine accessions were characterized as resistant (R) or moderately resistant (MR) and the rest were susceptible. A more detailed study with 20 selected (9 resistant and 11 other) accessions using the artificial inoculation method revealed that the accessions were significantly (p = 0.01) different in their reaction to the disease.
Pages 822-830 | Full Text PDF
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Assessment of popping ability of new tropical popcorn hybrids
Phumelele Jele, John Derera*, Muthulisi Siwela
School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa
KwaZulu-Natal Department of Agriculture and Environmental Affairs, South Africa
Summary
The objectives of the study were to determine variability among hybrids for popping quality traits, gene action involved in conditioning of quality traits in popcorn hybrids and relationships among quality traits in popcorn. Random crosses were generated among inbred lines resulting in 119 experimental F1 hybrids with adequate seed. A commercial hybrid P618 grown under the same conditions was used as a standard check. Significant variability was observed among hybrids for popping quality traits (p=0.05). The study identified 15 hybrids with superior popping quality. The hybrid 11POPH13 gave the highest flake volume (1 288 cm3) and the highest popping fold (25.75).
Pages 831-839 | Full Text PDF
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The effects of different concentrations of NAA on oil palm (Elaeis guineensis) embryoid cultures and phytosterols production
Zati Hanani Termizi*, Norrizah Jaafar Sidik, Tarmizi Ahmad Hashim, Norizan Ahmat
Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Malaysia
Tissue Culture Department, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi Bandar Baru Bangi, 43000 Kajang, Malaysia
Summary
This study aimed to investigate the effect of NAA concentrations on further improvement of oil palm (Elaeis guineensis) embryoid cultures of three selected Malaysian Palm Oil Board (MPOB) clones (PL 213, PL 209 and PL 220) and phytosterols production. The results indicated that PL 213 gave the highest mean of fresh weight (55.3±3.4 g) and number of shoots (123±4) in MS medium without NAA (control) followed by clones PL 209 and PL 220. There is a significant difference of clone and NAA concentration on the fresh weight of embryoid cultures but there is no significant effect of NAA concentration on the number of shoots.
Pages 840-847 | Full Text PDF
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Histological changes on regeneration in vitro culture of Date palm (Phoenix dactylifera) leaf explants
Ola H. Abd El Bar*and Maiada M. El Dawayati
Agriculture Botany Department, Faculty of Agriculture, Ain Shams University, 68 Hadayek Shoubra 11241, Cairo, Egypt
The Central Laboratory of Date Palm Researches and Development, Agriculture Research Center, Cairo, Egypt
Summary
A tissue culture experiment was conducted to investigate the effect of some growth regulators and two different ages of in vitro leaves of date palm c.v. sakoty on regeneration process. Leaves were cultured on Murashige and Skoogs medium (MS) supplemented with each or combined with the following growth regulators 4 mg l-1 2,4-D, 40 mg l-1 NAA, 2 mg l-1BA, (2 mg1 -1BA+ 40 mg l-1 NAA), (2mgl-1 BA+ 4 mg l-1 2,4-D) and (2 mg l-1BA+40 mg l-1 NAA+4 mg l-1 2,4-D). The results showed that in vitro young leaf explants treated with 2 mg1 -1 BA was more efficient in inducing direct somatic embryos and subsequent plantlets growth in a short time duration ,i.e.8-10 months.
Pages 848-855 | Full Text PDF
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Diagnosing nitrogen and sulfur status in marandu palisadegrass via non-destructive and destructive sampling methods
Karina Batista and Francisco Antonio Monteiro*
Instituto de Zootecnia, Secretaria de Agricultura e Abastecimento, Nova Odessa, SP, Brazil
Soil Science Department, Escola Superior de Agricultura Luiz de Queiroz, University of Sγo Paulo, Piracicaba, SP, Brazil
Summary
The objective of this study was to diagnose nitrogen and sulfur status in Brachiaria brizantha cv. Marandu via destructive and non-destructive sampling methods by growing this forage grass with different combinations of nitrogen and sulfur in a Quartzipsamment soil with low organic matter content. The results showed that diagnostic leaves of nitrogen-deficient marandu palisade grass plants had SPAD values of 16 to 20 and nitrogen concentrations of 11 to 14 g kg-1. Nitrogen-sufficient grasses had a SPAD value of =40-45 and nitrogen concentrations of >29.2 g kg-1. The sulfur status of grasses depended on their supply of nitrogen. Diagnostic leaves of plants treated with nitrogen but not sulfur had SPAD values of 17-28, sulfur concentrations of 0.6-1.2 g kg-1, sulfur-sulfate concentrations of 56-84 mg kg-1, and N:S ratios of up to 80:1.
Pages 856-865 | Full Text PDF
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Epistasis effects and inheritance of harvest index, drought and heat-resistance related traits in groundnut (Arachis hypogaea L.)
Chuni Lal*, Krishna Hariprasanna, Bharat Chikani, Harsukh Gor
Directorate of Groundnut Research, P. B. 5, Junagadh 362001, Gujarat, India
Directorate of Sorghum Research, Rajendranagar, Hyderabad 500030, India
Summary
Genetic control of harvest index (HI), and traits related to drought and heat resistance was studied following a triple test cross mating design. The inheritance pattern revealed importance of epistasis in the manifestation of all the traits. Partitioning of total epistasis to its component parts detected additive Χ additive (i) type epistasis for HI, and additive Χ dominance (j) + dominance Χ dominance (l) type for ?13C, however, for SCMR and RCI both i and j+l types of epistasis were important with the preponderance of the former. In two lines (JL 24 and PBS 12160), epistatic deviations were observed for SLA also when these were detected with the t-test.
Pages 866-872 | Full Text PDF | Supplementary data
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Bioassimilation behaviour of tomato seedling cultivars under different sources of artificial light
Eva Marνa Almansa, Antonio Espνn, Rosa Marνa Chica, Marνa Teresa Lao*
Agronomy Department of Higher Engineering School, University of Almeria, Ctra. Sacramento s/n, La Caρada de San Urbano, 04120, Almerνa, Spain
Higher Technical School of Civil Engineering,University of Granada, Campus Universitario de Fuentenueva, Edificio Politιcnico C/ Severo Ochoa s/n, 18071, Granada, Spain
Engineering Department of Higher Engineering School, University of Almerνa,Ctra. Sacramento s/n, La Caρada de San Urbano, 04120, Almerνa, Spain
Summary
The behaviour of fifteen tomato cultivars under different lights was carried out and quality of seedlings (biomass, plant structure, water status, carbohydrates starvation), was studied. The lamps used were compact fluorescent (184 W·m-2), high efficiency fluorescent (140 W·m-2) and tubular dons bulb fluorescent (108 W·m-2). Cultivars did not show the same behaviour under light treatments. The maximum response in biomass production of cultivars is obtained under high efficiency fluorescent treatment (16.56 W·m-2 PAR). For all cultivars, the relationship between total fresh weight and total dry weight presented the lowest value under high efficiency fluorescent treatment (low ratio, grate hardening). Starch quantity was higher than sugars for all treatments. Ikram cultivar has the greatest capacity to synthesize carbohydrates in all treatments.
Pages 873-880 | Full Text PDF
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Evaluation of some important woody plant species against wood destroying activity of honey fungus
Rahele Soltantoyeh*, Alireza Dalili, Ali Borhani
Damghan Islamic Azad University, Damghan, Iran
Agricultural and Natural Resources Research Center of Mazandaran, P.O. Box 48175-556, Sari, Iran
Summary
Armillaria mellea is one of the most important pathogens of plant species in horticultural and forest regions of Iran. In order to management of Armillaria root rot disease by tolerant plant species, resistant of 11 woody plants species was investigated against Armillaria mellea. The data analysis proved that Armillaria mellea isolates A23 and A1 caused the highest and lowest level of wood destroying activity with 11.82 and 7.11% wood weight loss, respectively. The tested plant indicated significant difference against the fungus. Citrus aurantium L., Ficus carica L., and Olea europaea L. with 19.00, 16.25 and 15.85% wood weight loss showed susceptible reaction to A. mellea. However, Zelkova carpinifolia Dippel with 1.68% wood weight loss were introduced as most tolerant species to A. mellea which followed by Prunus divaricate Ledeb. and Diospyrus lotus L., with 5.6 and 7.46% of wood weight loss, respectively.
Pages 881-886 | Full Text PDF
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Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.)
Sandra Mansur Scagliusi
EMBRAPA - Brazilian Agricultural Research Corporation, National Wheat Research Center, Passo Fundo - RS, 99001-970, Brazil
Summary
The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect.
Pages 887-894 | Full Text PDF
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Anatomy of vegetative organs and seed histochemistry of Physalis peruviana L.
Filipe Almendagna Rodrigues*, Joyce Dσria Rodrigues Soares, Renata Alves Lara Silva, Edwaldo dos Santos Penoni, Moacir Pasqual, Fabrνcio Josι Pereira, Evaristo Mauro de Castro
Tissue Culture Laboratory of the Department of Agriculture at Lavras Federal University, 37200-000, Lavras, Minas Gerais, Brazil
Laboratory of Plant Anatomy in the Department of Biology of Lavras Federal University, 37200-000, Lavras, Minas Gerais, Brazil
Summary
Physalis peruviana L. (Solanaceae) is a plant species whose fruits can be classified as fine fruits, which is similar to other species such as blueberry, raspberry, cherry, blackberry and pitaya. The objectives of the present study were to evaluate the type of reserve that occurs in seeds and anatomically characterize vegetative organs of Physalis peruviana L. (physalis). Histochemical tests and anatomical characterization were performed on the vegetative organs such as root, stem and leaf. Physalis seeds showed the presence of lipid reserves. The exposure of physalis to the east-west axis promoted thickening of the leaf midrib and blade and promoted the development of secondary xylem and phloem in the stem. Thus, the leaves on this axis appeared to show traits that favor greater radiation tolerance, which is a trait typical of sun leaves. The root of physalis has a uniseriate epidermis with the exodermis just below the epidermis.
Pages 895-900 | Full Text PDF
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Phenology, phyllochron, and gas exchanges in frigo and fresh strawberry (Fragaria Χ ananassa Duch.) plants of cv. Albion
Rosiani Castoldi da Costa*, Eunice Oliveira Calvete, Heloνsa Ferro Constβncio Mendonηa, Ana Paula Cecatto
University of Passo Fundo, Faculty of Agronomy and Veterinary Medicine, Campus I-Barrio San Josι - BR 285 - Km 171, CEP :99052-900, 611 Pillar Box - Passo Fundo, Rio Grande do Sul, Brazil
Summary
Aiming to identify a possible precocity in frigo and fresh strawberries plants of cv. Albion, in greenhouse, the cycle and the rate of leaf appearance were evaluated and the photosynthetic behavior of these plants was analyzed. Relating phenology evaluations and phyllochron determinations, frigo plants were more precocious than fresh plants. However, even frigo plants presenting a lower leaf emission rate, the thermal sum needed for the emission of successive leaves is lower (93.9 ΊC day leaf-1). Therefore, our study allows the producers, who want to sell strawberries in offseason, use frigo plants. However, fresh plants are recommended for those who cultivate in the traditional season.
Pages 901-908 | Full Text PDF
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Variation for infection response to Bipolaris sorokiniana and identification of trait specific sources in barley (Hordeum vulgare L.) germplasm
Tejveer Singh, Vinod K. Mishra*, Lal C. Prasad, Ramesh Chand
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Jhansi 284003, India
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Summary
The aim of present study was to evaluate un-adapted, exotic and indigenous germplasm of barley to identify useful genetic diversity for spot blotch resistance and other related traits to barley breeders. A total 124 genotypes were evaluated for spot blotch resistance and yield components, for three consecutive years at Varanasi, India. Based on Area Under Disease Progress Curve (AUDPC) score of spot blotch, three germplasm accessions (BCU422, BCU1204 and BCU5092) were found resistant to virulent isolate of spot blotch disease.
Pages 909-915 | Full Text PDF | Supplementary data
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QTL for grain yield under water stress and non-stress conditions over years in rice (Oryza sativa L.)
Sunil Kumar Verma*, Ritu Ravi Saxena, Ravi Ratna Saxena, Mary Suchita Xalxo, Satish Balkrishna Verulkar
Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur-492 012 Chhattisgarh, India
Department of Agriculture Statistics and Social Science, Indira Gandhi Krishi Vishwavidyalaya, Raipur-492 012 Chhattisgarh, India
Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur- 492 012 Chhattisgarh, India
Summary
A RIL population (F12 and F13 generation) developed from a cross between the two indica cultivars, Danteshwari (high yielding variety) and Dagad deshi (drought tolerant local land race). The population was phenotyped under combination of absolute rainfed (RF), terminal stage drought (TSD) and irrigated (I) conditions with direct seeding and transplanting during 2011 and 2012 to identify QTL for grain yield. Genotyping of all 122 RIL lines were done using 162 polymorphic SSR and HvSSR markers. The phenotypic and genotypic data was analyzed using QTL cartographer 2.5 and QTL IciMapping 3.2. The recombinant inbred lines exhibited significant interaction with conditions. In general, the lines performing better under stress were poor under non-stress condition; however, two lines (#17 and 57) performed well under all sets of conditions.
Pages 916-926 | Full Text PDF | Supplementary data
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Validation of SSR markers linked to the rust and late leaf spot diseases resistance in diverse peanut genotypes
Kanagee Navinbhai Gajjar, Gyan Prakash Mishra, Thankappan Radhakrishnan*, Sneha Meramanbhai Dodia, Arulthambi Luke Rathnakumar, Narendra Kumar, Santosh Kumar, Jentilal Ramjibhai Dobaria, Abhay Kumar
Directorate of Groundnut Research, Post Box No. 05, Junagadh- 362 001, Gujarat, India
Summary
In the present investigation, 22 SSR markers linked to rust and LLS diseases resistance were tested on 95 diverse genotypes for marker validation, of which 16 SSRs could be validated. Among rust resistant varieties and germplasm lines, nearly perfect marker validation was recorded but for 30 wild Arachis species, marker validation was very poor. Maximum numbers of rust and LLS linked-markers were found to be present on the linkage group 03 (eight SSRs) followed by linkage group 04 (three SSRs) which is an important step towards identification of more closely linked markers to the rust and LLS resistance QTLs and its cloning in future.
Pages 927-936 | Full Text PDF | Supplementary data
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Review article
Diversity assessment of sorghum germplasm and its utilization in genetic analysis of quantitative traits-A review
Tariq Shehzad* and Kazutoshi Okuno
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Japan 305-8572
Alliance for Research on North Africa (ARENA), University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Japan 305-8572
Summary
In this review, we describe the importance of genetic diversity in sorghum germplasm and its utilization in crop improvement through genetic analysis of important traits. The use of two different approaches of QTL mapping, namely family based linkage mapping and linkage disequilibrium based association mapping are discussed. This article surveys how QTL-based methods contribute to a better understanding of genetics mechanism involved in controlling phenotypic traits of interest. Some of the case studies of QTL mapping, controlling important traits in sorghum, are also reported in this review.
Pages 937-944 | Full Text PDF | Supplementary data
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Arbuscular mycorrhizal fungi (AMF) and phosphate-solubilizing fungus (PSF) on tolerance of beach plum (Prunus maritima) under salt stress
Zai Xueming*, Hao Zhenping, Zai Yu, Zhang Huanshi, Qin Pei
Horticulture Department, Jinling Institute of Technology, 130 village centers Qixia District, Nanjing, 210038, PR China
School of Pharmacy, Nanjing University of Chinese Medicine, 22 Xian-lin Avenne, Nanjing, 210046, PR China
Halophyte Research Laboratory, Nanjing University, 22 Han-kou Road, Nanjing, 210093, PR China
Summary
The synergic effect of an arbuscular mycorrhizal fungus (AMF) and a phosphate-solubilizing fungus (PSF) on the salt-tolerance of beach plum (Prunus maritima) grown in pots was explored. Pot experiments in greenhouse were carried out in one year beach plum inoculated with AMF (Funneliformis mosseae) or/and PSF (Mortierella sp. SM-1) under 1% NaCl stress. Salinity dramatically increased Na+ concentrations, but decreased K+ contents and K+/ Na+ in shoot and root significantly. And meanwhile, salinization reduced growth (root and shoot dry weight,total phosphate contents, chlorophyll contents) and biological soil quality (soil available phosphate contents and phosphatase enzyme activities).
Pages 945-950 | Full Text PDF
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Effect of Liquinox and Rosasol-N fertilizers on vegetative growth of phalaenopsis seedlings after transplantation into ex vitro conditions
I Gede Ketut Adiputra
Department of Biology, Faculty of Natural Science, University of Hindu Indonesia, Denpasar, Bali, Indonesia
Jl. Sangalangit, Tembau, Penatih, Denpasar, Bali, Indonesia
Summary
Ex vitro experiments were conducted to study the effect of NAA containing fertilizer (Liquinox) and high N containing fertilizer (Rosasol-N) on growth rate, chlorophyll content and viability of phalaenopsis seedlings previously micro propagated in vitro. This study found that during 46 days period of growth in ex vitro conditions, seedlings added Rosasol-N underwent both substantial decreases in growth rate and chlorophyll contents. In Liquinox added plants, whilst growth rate was slightly decreased, chlorophyll content was found substantially decreased.
Pages 951-956 | Full Text PDF
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Nitrogen use efficiency is associated with chlorophyll content in Brazilian spring wheat
Cristiano Lemes da Silva, Giovani Benin*, Elesandro Bornhofen, Eduardo Beche, Matheus Henrique Todeschini, Anderson Simionato Milioli
Agronomy Graduate Program, Federal University of technology - Paranα, Pato Branco - PR, CEP 85501-970, Brazil
Agronomy Course, Federal University of technology - Paranα, Pato Branco - PR, CEP 85501-970, Brazil
Summary
The objective of this study was to evaluate the general and specific combining ability (GCA and SCA) of agronomic traits associated with NUE components in a diallel cross. Six wheat parents and fifteen F2 populations were planted in the field in a randomized block design with three replications. The relative chlorophyll content was measured in flag leaves at the post-anthesis stage, while agronomic traits and NUE components were evaluated at physiological maturity. Additive and non-additive genetic effects were identified controlling NUE components.
Pages 957-964 | Full Text PDF
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Sugarcane cultivars present differential susceptibility to herbicides ametryn and trifloxysulfuron-sodium
Alexandre Ferreira da Silva, Leandro Galon, Germani Concenηo*, Ignacio Aspiazϊ, Evander Alves Ferreira, Siumar Pedro Tironi, Antonio Alberto da Silva
Brazilian Agricultural Research Corporation (EMBRAPA) Maize and Sorghum, Sinop - MT, Brazil
Federal University of the South Border (UFFS), Erechim - RS, Brazil
Brazilian Agricultural Research Corporation (EMBRAPA) Western Region Agriculture, Dourados - MS, Brazil
State University of Montes Claros (UNIMONTES), Janaϊba - MG, Brazil
PNPD-CAPES Fellowship, Jequitinhonha and Mucuri Valleys Federal University, Diamantina - MG, Brazil
Federal University of Alagoas, Maceiσ - AL, Brazil
Federal University of Viηosa, Viηosa - MG, Brazil
Summary
We aimed this study to evaluate the influence of the selective herbicides ametryn, trifloxysulfurom-sodium and its commercially available mixture on the growth of sugarcane cultivars. The results showed that trifloxysulfuron-sodium causes lower impact on all variables; therefore, being more selective to all cultivars. The label dose (1.0x), equivalent to 2,000 g ha-1 of ametryn and 22.5 g ha-1 of trifloxysulfuron-sodium is considered as a threshold between deficient weed control (in lower doses) and excessive crop toxicity (in higher doses). RB867515 and RB855113, respectively, were the most and least tolerant cultivars to both herbicides. In highly infested planting fields, farmers usually prefer to grow the most tolerant sugarcane cultivar when applying ametryn or trifloxysulfurom-sodium.
Pages 965-972 | Full Text PDF
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Proteome analysis of wheat (Triticum aestivum) for the identification of differentially expressed heat-responsive proteins
Ranjeet Ranjan Kumar*, Gyanendra Pratap Singh, Suneha Goswami, Himanshu Pathak, Raj Deo Rai
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India Pin 110012
Division of Genetics, Indian Agricultural Research Institute, New Delhi, India Pin 110012
CESCRA, Indian Agricultural Research Institute, New Delhi, India Pin 110012
Summary
We performed an extensive identification of heat-responsive proteins in control (22°C) and heat shock (HS) treated (42°C, 2 h) leaf samples of HD2985 cultivar of wheat at milky-ripe stage using 2-DE coupled with MALDITOF/MS, nLC/MS and MS2. Orbitrap was used because of its highest rate of true protein identification and speedy processing of samples. We identified 47 and 38 unique protein spots with 19 differentially expressed in control and heat shock (HS) treated samples.
Pages 973-986 | Full Text PDF | Supplementary data PDF | Supplementary data XLS
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Transferability of microsatellite loci in Coffea canephora
Ludymila Brandγo Motta, Taνs Cristina Bastos Soares*, Maria Amιlia Gava Ferrγo, Eveline Teixeira Caixeta, Ronald Martins Pereira Jϊnior, Rodrigo Monte Lorenzoni
Doutoranda do Programa de Pσs-Graduaηγo em Produηγo Vegetal, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Departamento de Farmαcia e Nutriηγo, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Embrapa Cafι/Incaper, Vitσria , ES, 29052-010, Brasil
Embrapa Cafι, BIOAGRO, BIOCAFΙ, Universidade Federal de Viηosa, Viηosa, MG, 36570-000, Brasil
Graduando em Agronomia, Centro de Ciκncias Agrαrias da Universidade Federal do Espνrito Santo, Alegre, ES, 29500-000, Brasil
Summary
We tested the heterologous amplification of 71 microsatellite markers, originally developed for Coffea arabica, in Coffea canephora. Heterologous amplification occurred with 38 of the 71 primers. Approximately 52.63% of the primers used were polymorphic; 35% of the primer sets were classified as non-informative, 50% as moderately informative and 15% as highly informative. The loci evaluated by SSRCa 088, M793, SSRCa 054, SSRCa 085, CM5, M25, CFGA54 were low informative; SSRCa 084, SSRCa 052 and SSRCa 040 were highly informative. The validated primer sets can be used in molecular studies for different purposes once they were efficient in the genotypes discrimination.
Pages 987-991 | Full Text PDF | Supplementary data
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Quality control to seeding systems and densities in peanut crop
Cristiano Zerbato*, Carlos Eduardo Angeli Furlani, Murilo Aparecido Voltarelli, Rafael Scabello Bertonha, Rouverson Pereira da Silva
Department of Rural Engineering, Universidade Estadual Paulista-UNESP/FCAV, Prof. Paulo Donato Castellane Access way, 14884-900 - Jaboticabal, SP-Brazil
Summary
The objective of this study was to use Statistical Process Control (SPC) to evaluate the quality of the operation of peanut seeding systems and densities. The parameters included the number of days to emergence, the longitudinal distribution of seedlings, the initial stand of plants and the crop yield. The seeding systems maintained a quality standard for the average number of days to seedling emergence. Compared to the longitudinal distribution of the seedlings in the control group, the mechanised seedings presented higher percentage of flawed spacing, which did not affect the crop yield.
Pages 992-998 | Full Text PDF
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